Gaddafi’s message, which was read on Syria’s al-Rai TV on Monday, accused revolutionary fighters of surrendering the oil-rich country to foreign occupiers.
“We will not hand Libya to colonialism, once again, as the traitors want,” said the brief statement attributed to Gaddafi.
“We will not be ruled after were the masters.”
He further described his rivals as traitors and urged his loyalists to press ahead with their fight across the North African country.
The remarks come as Gaddafi loyalists launched surprise fightbacks on several fronts on Monday.
Bani Walid is one of the four towns still controlled by Gaddafi supporters. The other towns are Jufrah, Sabha and Sirte.
Gaddafi’s whereabouts have been unknown since Tripoli fell into the hands of revolutionary forces and his 42-year dictatorship collapsed nearly three weeks ago.
Libya has been the scene of intense fighting between forces loyal to Gaddafi and opposition fighters since a revolution, seeking to topple Gaddafi, began in mid-February.